bf93ae2-dirty · Sep 3, 04:17 PM

based on the tnpb isdra nuclease, design an improved version with a more tolerant tam (not just ttgat)

A real build, finished August 31, 2026. Every number, model and refusal below is the record of that run - nothing here is a mock-up.

“based on the tnpb isdra nuclease, design an improved version with a more tolerant tam (not just ttgat)”

Use the steps on the left to read what was asked and what was chosen.

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Report · 20 min · measured here: 4 of 7

Ready to test

The design is ready.

3 requirements remain for the laboratory, as designated at the outset.

From here: the sequences are below, together with what the laboratory settles.

0did not come through

3for the laboratory

0accepted unmet

0faults still standing

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cand-001

Main sequence · 408 letters

MIRNKAFVVRLYPNAAQTELINRTLGSARFVYNHFLARRIAAYKESGKGLTFGQTSKELSLLRKAEETSWLNEVPSSALQNSLKNLETAYKNFFRTVKQSGKKVGFPRFRKKRTGESYRTQFVNNNIQIGEGRLKLPKLGWVKTKGQQDIQGKILNVTVRRIHEGHYEASVLCEVEIPYLPAAPKFAAGVDVGIKDFAIVTDGVRFKHEQNPKYYRSTLKRLRKAQQTLSRRKKGSARYGKAKTKLARIHKRIVNKRQDFLHKLTTSLVREYEIIGTEHLKPDNMRKNRRLALSISDAGWGEFIRQLEYKAAWYGRLVSKVSPYFPSSQLCHDCGFKNPEVKNLAVRTWTCPNCGETHDRDENAALNIRREALVAAGISDTLNAHGGYVRPASAGNGLRSENHATLVV

These exact letters appear in a file an instrument produced.

guide rna · 102 lettersGGUGGCUGCGGGAAUCUCAGACACCUUAAACGCUCAUGGAGGCUAUAUGGUCUGCGAAGUGAGAAUCACGCGACGUCGUGUGAGGUUCAAGAGUCCCUUGGCThese exact letters appear in a file an instrument produced.

target dna · 22 lettersGCCAAGGGACTCATCAATAGAAThese exact letters appear in a file an instrument produced.

non-target dna · 10 lettersTTCTATTGATThese exact letters appear in a file an instrument produced.

Make a set of this design

Two dozen versions of this exact shape, each folded and laid over the design above. Enough to fill a plate.

Some kept close to it
Some rewritten more freely
Every one ranked, with the letters ready to order

These were screened, not fully checked. Each one was folded and laid over the design it came from. They were NOT measured against this campaign’s requirements — only the design they came from was.

About three quarters of an hour. It keeps going with this page closed.

… and anything else you want to knowYou have used all 0 questions on this build.

Still unclear? Write to daniel@vidika.bio - a person reads it.

What next?

Two others were drawn for this one. Neither was built.

A clean start. Nothing from this run comes with you.

Since build 4

Requirement	Build 4	This build
The design must not match any known harmful sequence, such as a toxin or a disease-causing gene.	pass passed	pass passed
Maintains cutting activity at TTGAT sites at no less than the original TnpB IsDra nuclease.	0 passed	0 passed
Recognizes and cuts at TAM sequences beyond TTGAT.	8 passed	9 passed
The design’s own letters fold into the shape it was built as, rather than into some other shape.	1.94 passed	0.8924264705882348 passed
The design’s own letters fold into the shape it was built as, rather than into some other shape.	1.94 passed	1.95 passed

Only requirements that carried over unchanged appear here - where a requirement was reworded or replaced, there is nothing to compare it against and nothing is shown.